H2AX phosphorylation in A549 cells induced by the bulky and

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Antibody:Affinity purified rabbit anti-gamma-H2AX antibody A300-081A-T (lot A300-081A-T-21) used for WB at 1:1000. Detection:Chemiluminescence with an Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated by ATM and ATR MA1-2022 detects human and mouse phosphorylated H2AX. MA1-2022 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. Analysis of radiation-induced gamma-H2AX phosphorylation by western blot Molecular and Clinical Radiobiology Workshop McGill University Health Centre, June 17-19, 2015 ! Jose Joao Mansure, PhD, CCRP Research Associate, Research Institute, McGill University jose.mansure@mail.mcgill.ca Phospho-gamma H2AX pSer139 Antibody Product Specific Information General Information PA1-25001 detects gamma H2A.X (phospho S139) from human and mouse samples.

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A. Solutions and Reagents 2010-02-04 Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Invitrogen Anti-Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal (RM224), Catalog # MA5-33062. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications. This antibody reacts with All, Human, Mouse, Rat samples.

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Samples: Whole cell lysate (15 µg) from Jurkat cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody A300-081A (lot A300-081A-19) used for WB at 0.1 µg/ml.

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Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion. γ-H2AX can be detected by immunoblotting and immunostaining using microscopic or flow cytometric detection. Western blot and immunocytochemical analyses with the anti-gamma-H2AX antibody revealed that H2AX is phosphorylated at M phase in HeLa cells. In ataxia-telangiectasia cells lacking ATM kinase activity, gamma-H2AX was scarcely detectable in the mitotic chromosomes, suggesting involvement of ATM in M-phase phosphorylation of H2AX. Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4).

Bethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µL purified antibody (1 mg/mL). Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/ml Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cells treated with 100 µM etoposide, whole cell lysate The attached is a γH2AX western blotting result.293T cells were treated with etoposide or DMSO (control),then cells were lysed in RIPA buffer with sonication.0.2 nitrocelluose membrane was used I don't do anything unusual for the western blot except use a 12% gel and be careful not to run H2A.X off the gel since it's small. If you can see a MW marker of similar size on your membrane, the Western blot and immunocytochemical analyses with the anti-gamma-H2AX antibody revealed that H2AX is phosphorylated at M phase in HeLa cells.
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study from North America and western Europe) and on experimental with BSA: γ-H2AX generation not after (Western Blot); 50-250 µg/ml.

Gamma H2AX is the phosphorylated form 2009-12-10 Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated by ATM and ATR 754 gamma H2AX antibodies from 38 antibody suppliers Click each gamma H2AX antibody that interests you to view the full gamma H2AX antibody datasheet.
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Bethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µL purified antibody (1 mg/mL). Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/ml Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cells treated with 100 µM etoposide, whole cell lysate The attached is a γH2AX western blotting result.293T cells were treated with etoposide or DMSO (control),then cells were lysed in RIPA buffer with sonication.0.2 nitrocelluose membrane was used I don't do anything unusual for the western blot except use a 12% gel and be careful not to run H2A.X off the gel since it's small. If you can see a MW marker of similar size on your membrane, the Western blot and immunocytochemical analyses with the anti-gamma-H2AX antibody revealed that H2AX is phosphorylated at M phase in HeLa cells.